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Other publications by Jean-Christophe Nebel

Identification of NAD(P)H Quinone Oxidoreductase Activity in Azoreductases from P. aeruginosa: Azoreductases and NAD(P)H Quinone Oxidoreductases Belong to the Same FMN-Dependent Superfamily of Enzymes

A. Ryan, E. Kaplan, J.-C. Nebel, E. Polycarpou, V. Crescente, E. Lowe, G. Preston & E. Sim

PLOS ONE
10.1371/journal.pone.0098551, 2014
[PDF]

Abstract
Water soluble quinones are a group of cytotoxic anti-bacterial compounds that are secreted by many species of plants, invertebrates, fungi and bacteria. Studies in a number of species have shown the importance of quinones in response to pathogenic bacteria of the genus Pseudomonas. Two electron reduction is an important mechanism of quinone detoxification as it generates the less toxic quinol. In most organisms this reaction is carried out by a group of flavoenzymes known as NAD(P)H quinone oxidoreductases. Azoreductases have previously been separate from this group, however using azoreductases from Pseudomonas aeruginosa we show that they can rapidly reduce quinones. Azoreductases from the same organism are also shown to have distinct substrate specificity profiles allowing them to reduce a wide range of quinones. The azoreductase family is also shown to be more extensive than originally thought, due to the large sequence divergence amongst its members. As both NAD(P)H quinone oxidoreductases and azoreductases have related reaction mechanisms it is proposed that they form an enzyme superfamily. The ubiquitous and diverse nature of azoreductases alongside their broad substrate specificity, indicates they play a wide role in cellular survival under adverse conditions.

Cited by ( Google Scholar: 54, ISI Web of Knowledge: 33 & SCOPUS: 28 ): 61

2024

The dynamic crosslinking between gut microbiota and inflammation during aging: reviewing the nutritional and hormetic approaches against dysbiosis and inflammaging, Sakshi Chaudhary, Pardeep Kaur, Thokchom Arjun Singh, Kaniz Shahar Bano, Ashish Vyas, Alok Kumar Mishra, Prabhakar Singh, Mohammad Murtaza Mehdi, Biogerontology 26(1):1-40, DOI: 10.1007/s10522-024-10146-2, 2024 (G)
Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders in Acinetobacter pittii, Elise M Van Fossen, Jared O Kroll, Lindsey N Anderson, Andrew D McNaughton, Daisy Herrera, Yasuhiro Oda, Andrew J Wilson, William C Nelson, Neeraj Kumar, Andrew R Frank, Joshua R Elmore, Pubudu Handakumbura, Vivian S Lin, Robert G Egbert, Applied and Environmental Microbiology, DOI: 10.1128/aem.01026-24, 2024 (G) (I)
Mining the Metabolic Capacity of Clostridium sporogenes Aided by Machine Learning Huanrong Ouyang, Zhao Xu, Joshua Hong, Jeshua Malroy, Liangyu Qian, Shuiwang Ji, Xuejun Zhu, Angewandte Chemie International Edition, https://doi.org/10.1002/anie.202319925, 2024 (G) (I)
Sequence similarity network analysis of drug-and dye-modifying azoreductase enzymes found in the human gut microbiome, Audrey R. Long, Emma L. Mortara, Brisa N. Mendoza, Emma C. Fink, Francis X. Sacco, Matthew J. Ciesla, Tyler.M.M. Stack, Archives of Biochemistry, https://doi.org/10.1016/j.abb.2024.110025, 2024 (G) (I)

2023

Leaf proteomics of sugarcane inoculated with growth-promoting rhizobacterium and fertilized with molybdenum, Valeska Regina Silva Martins Mendes, Emidio Cantidio Almeida de Oliveira, Larissa Batista da Silva, Lucas Carvalho de Freitas, Amanda Michele Santos de Lima, Fabiana Aparecida Cavalcante Silva, Tercilio Calsa Junior, Fernando Jose Freire, Plant and Soil, DOI: 10.1007/s11104-023-06379-8, 2023 (G) (I)
The global anaerobic metabolism regulator fnr is necessary for the degradation of food dyes and drugs by Escherichia coli, Lindsey M. Pieper, Peter Spanogiannopoulos, Regan F. Volk, Carson J. Miller, Aaron T. Wright, Peter J. Turnbaugh, Human Microbiome, 14(5), 2023 (G) (I)
Intestine Offers Board and Lodging for Intestinal Microbes on a Short-or Long-Term Stay, F Blachier, In: Metabolism of Alimentary Compounds by the Intestinal Microbiota and Health. Springer, Cham. https://doi.org/10.1007/978-3-031-26322-4_2, 2023 (G)
Identification of genes responding in apple replant disease (ARD) affected Malus roots using an RNA-sequencing approach, S. Reim, A. Cestaro, T. Winkelmann, H. Flachowsky, Acta Horticulturae, DOI: 10.17660/ActaHortic.2023.1362.13, 2023
Role of biofilms in hospital-acquired infections (HAIs), Shraddha Sharma, Vaishali Kaushik, Vishvanath Tiwari, In book: Understanding Microbial Biofilms, DOI: 10.1016/B978-0-323-99977-9.00001-6, 2023
Research progress on adaptive modifications of the gut microflora and regulation of host glucose and lipid metabolism by cold stimulation, Wanting Wei, Guanyu Zhang, Yongqiang Zhang, Li Zhang, Shuai Wu, Xi Li, Danfeng Yang, Frigid Zone Medicine, 3(1):13-21, DOI: 10.2478/fzm-2023-0003, 2023 (G)

2022

Roles of Ferredoxin-NADP+ Oxidoreductase and Flavodoxin in NAD (P) H-Dependent Electron Transfer Systems, T Iyanagi, Antioxidants, 11(11), 2143, https://doi.org/10.3390/antiox11112143, 2022 (G) (I)
Xenobiotics and drug-metabolizing enzymes: Challenges and strategies, Yasra Sayyed, Kanwal Rehman, Muhammad Sajid Hamid Akash, Sairah Hafeez Kamran, Mohammad Badran, In book: Biochemistry of Drug Metabolizing Enzymes, DOI: 10.1016/B978-0-323-95120-3.00007-5, 2022
Gut microbiome-wide search for bacterial azoreductases reveals potentially uncharacterized azoreductases encoded in the human gut microbiome, Domenick J. Braccia, Glory Minabou Ndjite, Ashley Weiss, Sophia Levy, Stephenie Abeysinghe, Xiaofang Jiang, Mihai Pop, Brantley Hall, Drug Metabolism and Disposition: the Biological Fate of Chemicals, DOI: 10.1124/dmd.122.000898, 2022 (G) (I)
Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system, Deepak Rawat, Udita Sharma, Pankaj Poria, Arran Finlan, Brenda Parker, Radhey Shyam Sharma, Vandana Mishra, ISME Communications, DOI: 10.1038/s43705-022-00161-0, 2022
Proteomic analysis of Burkholderia zhejiangensis CEIB S4–3 during the methyl parathion degradation process, Maria Luisa Castrejon-Godinez, Efraín Tovar-Sanchez, Ma. Laura Ortiz-Hernandez, Sergio Encarnacion-Guevara, Angel Gabriel Martínez Batallar, Magdalena Hernandez Ortiz, Enrique Sanchez-Salinas, Alexis Joavany Rodriguez, Patricia Mussali-Galante, Pesticide Biochemistry and Physiology, 187, 105197, DOI: 10.1016/j.pestbp.2022.105197, 2022 (G) (I)
Chemical-damage MINE: A database of curated and predicted spontaneous metabolic reactions, James G.Jeffryes, Claudia Lerma-Ortiz, Filipe Liu, Alexey Golubev, Thomas D.Niehaus, Mona Elbadawi-Sidhu, Oliver Fiehn, Andrew D.Hanson, Keith EJ.Tyo, Christopher S.Henry, Metabolic Engineering, Volume 69, January 2022, Pages 302-312, https://doi.org/10.1016/j.ymben.2021.11.009, 2022 (G)
Drug-metabolizing enzymes and fate of prodrugs: From function to regulation, TH Mallhi, MH Butt, A Ahmad et al., in book Biochemistry of Drug Metabolizing Enzymes: Trends and Challenges, 2022 (G)
Identification of molecular basis that underlie enzymatic specificity of AzoRo from Rhodococcus opacus 1CP: A potential NADH:quinone oxidoreductase, Anna Christina R. Ngo, Jingxian Qi, Cindy Juric, Isabel Bento, Dirk Tischler, Archives of Biochemistry and Biophysics, Volume 717, DOI: 10.1016/j.abb.2022.109123, 2022 (G) (S)
Human Gut Microbiota and Drug Metabolism, Archana Pant, Tushar K. Maiti, Dinesh Mahajan, Bhabatosh Das, Microbial Ecology, DOI: 10.1007/s00248-022-02081-x, 2022 (G)

2021

MdaB and NfrA, two novel reductases important in the survival and persistence of the major enteropathogen Campylobacter jejuni, F Nasher, AJ Taylor, A Elmi, B Lehri, UZ Ijaz, D Baker, R Goram, S Lynham, D Singh, R Stabler, DJ Kelly, O Gundogdu, BW Wren, Journal of Bacteriology, 204(1), art. no. e00421-21, doi:10.1128/JB.00421-21, 2021 (G) (S)
Delineating the potential targets of thymoquinone in ESKAPE pathogens using a computational approach, A. S. Smiline Girija, S. Gnanendra, A. Paramasivam, J. Vijayashree Priyadharsini, In Silico Pharmacology, 9(1), DOI: 10.1007/s40203-021-00111-z, 2021
Proteomic Analyses of Acinetobacter baumannii Clinical Isolates to Identify Drug Resistant Mechanism, Ping Wang, Ren-Qing Li, Lei Wang, Wen-Tao Yang, Qing-Hua Zou and Di Xiao, Front Cell Infect Microbiol., 11: 625430, doi: 10.3389/fcimb.2021.625430, 2021 (G)
Gut microbiota mediates the absorption of FLZ, a new drug for Parkinson's disease treatment, J Shang, S Ma, C Zang, X Bao, Y Wang, D Zhang, Pharmaceutica Sinica B, https://doi.org/10.1016/j.apsb.2021.01.009, 2021 (G) (S)
Mechanisms of Azoreduction by the Human Gut Microbiome, Hitchings, Reese D., PhD Thesis, Albert Einstein College of Medicine, New York, 2021 (G)

2020

Comparative genome analyses of Mycobacteroides immunogenum reveals two potential novel subspecies, Siew Woh Choo, Shusruto Rishik, Wei Yee Wee, Microbial Genomics, 6(12), DOI: 10.1099/mgen.0.000495, 2020
BIOTRANSFORMATION OF THE XENOBIOTICS BY MICROBIOTA OF THE GASTROINTESTINAL TRACT AND ITS CONSEQUENCES FOR HUMANS, Galkin BM, Filipova TO, Microbiology & Biotechnology, DOI: 10.18524/2307-4663.2020.2(49).211285, 2020 (G)
Altered proteome of a Burkholderia pseudomallei mutant defective in short-chain dehydrogenase affects cell adhesion, biofilm formation and heat stress tolerance, Reamtong O, Indrawattana N, Rungruengkitkun A, Thiangtrongjit T, Duangurai T, Chongsa-nguan M, Pumirat P. PeerJ 8:e8659 https://doi.org/10.7717/peerj.8659, 2020 (G)
Mechanistic and Crystallographic Studies of Azoreductase AzoA from Bacillus wakoensis A01, Elvira Romero, Simone Savino, Marco W Fraaije, Nikola Loncar, ACS Chemical Biology, DOI: 10.1021/acschembio.9b00970, 2020 (G) (I) (S)
Biotransformation of the xenobiotics by microbiota of the gastrointestinal tract and its consequences for humans, Boris Mikolayovich Galkin, Tatiana Olegovna Filipova, Microbiology and biotechnology, Odessa National University of Men І. I. Mechnikov, DOI: http://dx.doi.org/10.18524/2307-4663.2020.2(49).211285, 2020 (G)

2019

Tri-phasic engineered wetland system for effective treatment of azo dye-based wastewater, Dileep Kumar Yeruva, Palle Ranadheer, A. Kiran Kumar, S Venkata Mohan, DOI: 10.1038/s41545-019-0037-y, 2019 (G) (I)
Kinetic Investigation of a Presumed Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes a New Class of NAD(P)H:quinone Reductases, Renata Almeida Garcia Reis, Francesca Salvi, Isabella WIlliams, and Giovanni Gadda, Biochemistry, DOI: 10.1021/acs.biochem.9b00207, 2019 (G) (I) (S)
The Importance of Anthraquinone and Its Analogues and Molecular Docking Calculation, Sefa Celik, Funda Ozkok, Sevim Akyuz, Aysen E. Ozel, In book: Computational Models for Biomedical Reasoning and Problem Solving, DOI: 10.4018/978-1-5225-7467-5.ch007, 2019 (G)
Remarkable diversification of bacterial azoreductases: primary sequences, structures, substrates, physiological roles, and biotechnological applications, Hirokazu Suzuki, Applied Microbiology and Biotechnology, DOI: 10.1007/s00253-019-09775-2, 2019 (G) (I) (S)
Biodegradation of azo dye using beneficial microorganisms and transcriptomic analysis of the degradation pathway, Chaeyoung Rhee, MSc Thesis, Korea Maritime and Ocean University, 2019 (G)

2018

Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34melT, María Elisa Pavan, Esmeralda Solar Venero, Diego E. Egoburo, Esteban E. Pavan, Nancy I. Lopez, M. Julia Pettinari, Applied microbial and cell physiology, 2018 (G) (I) (S)
Steric hindrance controls pyridine nucleotide specificity of a flavin‐dependent NADH: quinone oxidoreductase, J Ball, RAG Reis, J Agniswamy, IT Weber, G Gadda, Protein Science, https://doi.org/10.1002/pro.3514, 2018 (G) (I) (S)
Symbiotic characteristics of Bradyrhizobium diazoefficiensUSDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max), Yuan Hui Liu, En Tao Wang, Yin Shan Jiao, Chang Fu Tian, Lei Wang, Zi Jian Wang, Jia Jing Guan, Raghvendra Pratap Singh, Wen Xin Chen, Wen Feng Chen, Microbiological Research, Volume 214, Pages 19–27, 2018 (G) (I) (S)
Microbial Degradation of Azo Dyes: The Role of Azoreductase to Initiate Degradation, Dirk Tischler, Jingxian Qi, Anna Christina R. Ngo and Michael Schloemann, Handbook of Research on Microbial Tools for Environmental Waste Management, Pages: 31, DOI: 10.4018/978-1-5225-3540-9.ch016, 2018 (G)
Kinetic Characterization of PA1225 from Pseudomonas aeruginosa PAO1 Reveals a New NADPH:Quinone Reductase, Elias Flores and Giovanni Gadda, Biochemistry, DOI: 10.1021/acs.biochem.8b00090, 2018 (G) (I) (S)
Azoreductase: a key player of xenobiotic metabolism, Santosh A. Misal, Kachru R. Gawai, Bioresour. Bioprocess, 5:17, doi: 10.1186/s40643-018-0206-8, 2018 (G) (I) (S)

2017

Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida, Rinu Kooliyottil, Louise-Marie Dandurand, Joseph C. Kuhl, Allan Caplan and Fangming Xiao, Plant Methods, 13:68, 2017 (G) (I) (S)
Methionine Residues in Exoproteins and Their Recycling by Methionine Sulfoxide Reductase AB Serve as an Antioxidant Strategy in Bacillus cereus, Jean-Paul Madeira, Beatrice M. Alpha-Bazin, Jean Armengaud, Catherine Duport, Frontiers in Microbiology 8:1342, 2017 (G) (I) (S)
Diverse Molecular Resistance Mechanisms of Bacillus megatherium During Metal Removal Present in a Spent Catalyst, Andrea Rivas-Castillo, Domancar Luka Orona-Tamayo, Gomez Ramirez, Norma G. Rojas-Avelizapa, Biotechnology and Bioprocess Engineering, 22(3), 2017 (G) (I) (S)
Chemical transformation of xenobiotics by the human gut microbiota N Koppel, VM Rekdal, EP Balskus, Science, Vol. 356, Issue 6344, 2017 (G) (I) (S)
Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity, Valérie Chalansonnet, Claire Mercier, Sylvain Orenga and Christophe Gilbert, BMC Microbiology, 17:126, 2017 (G) (I) (S)
Antibacterial activity, computational analysis and host toxicity study of thymol-sulfonamide conjugates SS Swain, SK Paidesetty, RN Padhy, Biomedicine & Pharmacotherapy, 88:181-193, 2017 (G) (I) (S)
Structure, biochemical and kinetic properties of recombinant Pst2p from Saccharomyces cerevisiae, a FMN-dependent NAD(P)H:quinone oxidoreductase, Karin Koch, Altijana Hromic, Marija Sorokina, Emilia Strandback, Manuel Reisinger, Karl Gruber, Peter Macheroux, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2017 (G) (I) (S)
Cloning, Purification, and Biochemical Characterization of PA1225, a Novel FAD Containing NADPH:Quinone Reductase from Pseudomonas aeruginosa PAO1. Flores, Elias, MSc Thesis, Georgia State University, 2017 (G)

2016

Draft genome sequence of Paenibacillus sp. strain DMB5, acclimatized and enriched for catabolizing anthropogenic compounds, Johnson J, Shah B, Jain K, Parmar N, Hinsu A, Patel N, Joshi CG, Madamwar D., Genome Announc, 4(2):e00211-16, doi:10.1128/genomeA.00211-16, 2016 (G) (I) (S)
Azoreductases in drug metabolism, A Ryan, British journal of pharmacology, DOI: 10.1111/bph.13571, 2016 (G) (I) (S)
Time-resolved singlet-oxygen luminescence detection with an efficient and practical semiconductor single-photon detector, G Boso, D Ke, B Korzh, J Bouilloux, N Lange, and H Zbinden, Biomedical Optics Express, 7(1): 211-224, 2016 (G) (I) (S)
Identification of novel members of the bacterial azoreductase family in Pseudomonas aeruginosa, Vincenzo Crescente, Sinead M. Holland, Sapna Kashyap, Elena Polycarpou, Edith Sim, Ali Ryan, Biochemical Journal, 473(5):549-558, DOI: 10.1042/BJ20150856, 2016 (G) (I) (S)
Microbial Resistance to Natural Compounds: Challenges for Developing Novel Alternatives to Antibiotics, Ciubuca, Bianca-Maria; Saviuc, Crina-Maria; Chifiriuc, Mariana-Carmen; Lazar, Veronica, Current Organic Chemistry, 20(28):2983-2988(6), 2016 (G) (I) (S)
Functional Annotation of a Presumed Nitronate Monoxygenase Reveals a New Class of NADH: Quinone Reductases, J Ball, F Salvi, G Gadda, Journal of Biological Chemistry, doi: 10.1074/jbc.M116.739151, 2016 (G) (I) (S)

2015

Flavodoxin-Like Proteins Protect Candida albicans from Oxidative Stress and Promote Virulence, Lifang Li, Shamoon Naseem, Sahil Sharma, James B. Konopka, PLoS Pathog, 11(9): e1005147. doi:10.1371/journal.ppat.1005147, 2015 (G) (I) (S)
Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase, Vercammen K, Wei Q, Charlier D, Doetsch A, Hauessler S, Schulz S, Salvi F, Gadda G, Spain J, Rybtke ML, Tolker-Nielsen T, Dingemans J, Ye L, Cornelis P., J Bacteriol, 197:1026–1039, doi:10.1128/JB.01991-14, 2015 (G) (I) (S)
Draft genome sequence of Paenibacillus sp. strain DMB20, isolated from Alang ship-breaking yard, which harbors genes for xenobiotic degradation, Shah B, Jain K, Patel N, Pandit R, Patel A, Joshi CG, Madamwar D., Genome Announc, 3(3):e00554-15, doi:10.1128/genomeA.00554-15, 2015 (G) (S)
Multi-Enzymatic Systems for Cleaning-up Synthetic Dyes from the Environment, SAG Mendes, PhD Thesis, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Portugal, 2015 (G)
Biochemical characterization of inducible 'reductase'component of benzoate dioxygenase and phthalate isomer dioxygenases from Pseudomonas aeruginosa strain PP4, Karandikar, R., Badri, A. & Phale, P.S., Appl Biochem Biotechnol, 177:318, doi:10.1007/s12010-015-1744-6, 2015 (G) (I) (S)
Identification of novel factors that promote virulence of the human fungal pathogen Candida albicans, Lifang Li, PhD thesis in genetics, State University of New York, 2015 (G)

2014

Reviews in Environmental Science and Biotechnology, Imran, M.aEmail Author, Crowley, D.E.b, Khalid, A.c, Hussain, S.d, Mumtaz, M.W.a, Arshad, M.e, Microbial biotechnology for decolorization of textile wastewaters(Review), 14(1):73-92, 2014 (S)

j.nebel@kingston.ac.uk