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Other publications by Jean-Christophe Nebel

Primary sequence contribution to optical function of the eye lens

K. Mahendiran, C. Elie, J.-C. Nebel, A. Ryan and B.K. Pierscionek

Scientific Reports
4, 5195, 2014
[PDF]

Abstract
The crystallins have relatively high refractive increments compared to other proteins. The Greek key motif in beta/gamma-crystallins was compared with that in other proteins, using predictive analysis from a protein database, to see whether this may be related to the refractive increment. Crystallins with Greek keys motifs have significantly higher refractive increments and more salt bridges than other proteins with Greek key domains. Specific amino acid substitutions: lysine and glutamic acid residues are replaced by arginine and aspartic acid, respectively as refractive increment increases. These trends are also seen in S-crystallins suggesting that the primary sequence of crystallins may be specifically enriched with amino acids with appropriate values of refractive increment to meet optical requirements. Comparison of crystallins from five species: two aquatic and three terrestrial shows that the lysine/arginine correlation with refractive increment occurs in all species investigated. This may be linked with formation and maintenance of salt bridges.

Cited by ( Google Scholar: 36, ISI Web of Knowledge: 17 & SCOPUS: 16 ): 36

2024

The Functional Significance of High Cysteine Content in Eye Lens γ-Crystallins, E Serebryany, RW Martin, GR Takahashi, Biomolecules, 2024 (G) (I)
The importance of the fourth Greek key motif of human γD-crystallin in maintaining lens transparency—the tale told by the tail, VenkataPullaRao Vendra, Madhupreetha Thangapandian, Molecular Vision, 30:37-48, 2024 (G) (I)

2023

Eye Lens Organoids Made Simple: Characterization of a New Three-Dimensional Organoid Model for Lens Development and Pathology, Matthieu Duot, Roselyne Viel, Justine Viet, Catherine Le Goff-Gaillard, Luc Paillard, Salil A. Lachke, Carole Gautier-Courteille, David Reboutier, Cells, 12(20):2478, DOI: 10.3390/cells12202478, 2023 (G) (I)
Age-Dependent Changes in the Water Content and Optical Power of the In Vivo Mouse Lens Revealed by Multi-Parametric MRI and Optical Modeling, Xingzheng Pan, Eric R Muir, Caterina Sellitto, Kehao Wang, Catherine Cheng, Barbara Pierscionek, Paul J Donaldson, Thomas W White, Investigative ophthalmology & visual science, Investigative Ophthalmology & Visual Science, 64(4):24, DOI: 10.1167/iovs.64.4.24, 2023 (G) (I)
Illuminating Assembly Dynamics Regulating Short-Range Order Optics in Extremely Long-Lived Proteins, Michael Richard Bergman, Northeastern University, 2023 (G)
Brito, Catarina Manique da Silva, Avaliação da exposição ocupacional dos técnicos de medicina nuclear a radiações ionizantes na estrutura ocular. Lisboa: Instituto Superior de Engenharia de Lisboa. Dissertação de Mestrado, 2023 (G)

2022

Regulation of lens water content: Effects on the physiological optics of the lens, Progress in Retinal and Eye Research, Paul J. Donaldson, Yadi Chen, Rosica S. Petrova, Angus C. Grey, Julie C. Lim, DOI: 10.1016/j.preteyeres.2022.101152, 2022 (G) (I)
Mollusc Crystallins: Physical and Chemical Properties and Phylogenetic Analysis, Dominova, I.N., Zhukov, V.V., Diversity, 14, 827, https://doi.org/10.3390/d14100827, 2022 (G) (I)
Insights Into The Biochemical And Biophysical Mechanisms Mediating The Longevity Of The Transparent Optics Of The Eye Lens, RA Quinlan, JI Clark, Journal of Biological Chemistry, 2022 (G) (I)
Silymarin from Milk Thistle Fruits Counteracts Selected Pathological Changes in the Lenses of Type 1 Diabetic Rats, Weronika Borymska, Maria Zych, Slawomir Dudek, Ilona Kaczmarczyk-Sedlak, Nutrients 14(7):1450, DOI: 10.3390/nu14071450, 2022 (G)
Transcriptomic evidence for visual adaptation during the aquatic to terrestrial metamorphosis in leopard frogs, Ryan K. Schott, Rayna C. Bell, Ellis R. Loew, Kate N. Thomas, David J. Gower, Matthew K. Fujita, BMC Biology 20(1):138, DOI: 10.1186/s12915-022-01341-z, 2022 (G)

2021

Insights into the binding of morin to human γD-crystallin, Rodrigo Aguayo-Ortiz, Rodrigo Aguayo-Ortiz, Dulce C. Guzman-Ocampo, Laura Dominguez, Biophysical Chemistry, DOI: 10.1016/j.bpc.2021.106750, 2021 (G) (S)
Surface Exposed Free Cysteine Suppresses Crystallization of Human γD-Crystallin, Alessandro Strofaldi, Amir R. Khan, Jennifer J. McManus, Journal of Molecular Biology, 433(22):167252, DOI: 10.1016/j.jmb.2021.167252, 2021 (G)
Redox chemistry of lens crystallins: A system of cysteines, Eugene Serebryany, David C. Thorn, Liliana Quintanar, Experimental Eye Research, DOI: 10.1016/j.exer.2021.108707, 2021 (G)
Missense Mutations in the Gamma Crystallins and Mechanisms of Lens Opacity, Hou, Wenjuan, PhD Thesis, State University of New York at Albany, 2021 (G)
Genomic and Spectral Visual Adaptation in Southern Leopard Frogs during the Ontogenetic Transition from Aquatic to Terrestrial Light Environments, yan K Schott, Rayna C Bell, Ellis R Loew, Kate N Thomas, David J Gower, Jeffrey W Streicher, Matthew K Fujita, DOI: 10.1101/2021.02.19.432049, preprint, 2021 (G)

2020

Chemical Imaging of Retinal Pigment Epithelium in Frozen Sections of Zebrafish Larvae Using ToF-SIMS, Sebastiaan Van Nuffel, Khai C. Ang, Alex Y. Lin, and Keith C. Cheng, J. Am. Soc. Mass Spectrom., 2020 (G)
Cell compaction is not required for the development of gradient refractive index profiles in the embryonic chick lens, K Wang, M Hoshino, K Uesugi, N Yagi, R Young, B Frost, J Regini, A Quantock, B Pierscionek, Experimental Eye Research, 2020 (G) (S)
Two-Dimensional Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract, Paul C. Guest, In book: Clinical and Preclinical Models for Maximizing Healthspan, DOI: 10.1007/978-1-0716-0471-7_14, 2020 (G)
Tools to Evaluate Nanodiamond-Mediated Delivery of Tiopronin for Cataract Prevention, Justin Edward Beltz, PhD thesis, Missouri University of Science and Technology, 2020 (G)

2019

Effects of Mutating Trp42 Residue on γD-crystallin Stability, Rodrigo Aguayo-Ortiz, Laura Dominguez, J. Chem. Inf. Model., https://doi.org/10.1021/acs.jcim.9b00747, 2019 (G) (S)
Transcriptional profiling of single fiber cells in a transgenic paradigm of an inherited childhood cataract reveals absence of molecular heterogeneity, Suraj P. Bhat, Rajendra K. Gangalum, Dongjae Kim, Serghei Mangul, Raj K. Kashyap, Xinkai Zhou and David Elashoff, J. Biol. Chem., 2019 (G) (I) (S)
Thermodynamic Stability of Human γD-crystallin Mutants Using Alchemical Free Energy Calculations, Rodrigo Aguayo-Ortiz, Augusto Gonzalez-Navejas, Giovanni Palomino-Vizcaino, Oscar Rodriguez-Meza, Miguel Costas, Liliana Quintanar, Laura Dominguez, J. Phys. Chem. B2019 (G) (I) (S)
Alzheimer's Disease Is Responsible for Progressive Age-Dependent Differential Expression of Various Protein Cascades in Retina of Mice, Javed Iqbal, Kaoyuan Zhang, Na Jin, Yuxi Zhao, Xukun Liu, Qiong Liu, Jiazuan Ni, and Liming Shen, ACS Chemical Neuroscience, DOI: 10.1021/acschemneuro.8b00710, 2019 (G) (I) (S)

2018

Spatial Analysis of Single Fiber Cells of the Developing Ocular Lens Reveals Regulated Heterogeneity of Gene Expression, RK Gangalum, D Kim, RK Kashyap, S Mangul, X Zhou, D Elashoff, SP Bhat, iScience, Volume 10, Pages 66-79, 2018 (G) (I) (S)
Protein refractive index increment is determined by conformation as well as composition, Khago D., Bierma J.C., Roskamp K.W., Kozlyuk N., Martin R.W., Journal of Physics Condensed Matter, 30(43), 2018 (G) (I) (S)

2017

Rapid and Parallel Adaptive Evolution of the Visual System of Neotropical Midas Cichlid Fishes, Torres-Dowdall, J., Pierotti, M.E.R., Haerer, A., Molecular Biology and Evolution, 34(10), pp. 2469-2485, 2017 (G) (I) (S)
Crystal Structure of Chicken γS-Crystallin Reveals Lattice Contacts with Implications for Function in the Lens and the Evolution of the βγ-Crystallins, Sagar, V. , Chaturvedi, S.K. , Schuck, P., Structure, 25(7), pp. 1068-1078.e2, 2017 (G) (I) (S)
Identification of Sequence Similarities among Isomerization Hotspots in Crystallin Proteins, Lyon, Y.A. , Sabbah, G.M. , Julian, R.R., Journal of Proteome Research, 16(4), pp. 1797-1805, 2017 (G) (I) (S)
A Retrospective Analysis of Visual Outcomes in Laser Vision Correction of Hyperopic Patients Using the VISX STAR S4 IR® and the WaveLight® EX500 Excimer Laser Platforms, Nitz, Michael Allen, MSc Thesis, Boston University, 2017 (G)

2016

Theoretical approximations and experimental extinction coefficients of biopharmaceuticals, Miranda-Hernandez, M.P., Valle-Gonzalez, E.R., Ferreira-Gomez, D., Perez, N.O., Flores-Ortiz, L.F., Medina-Rivero, E., Analytical and Bioanalytical Chemistry, Volume 408, Issue 5, pp. 1523-1530, 2016 (G) (I) (S)
Gamma crystallins of the human eye lens, Vendra, V.P.R., Khan, I., Chandani, S., Muniyandi, A., Balasubramanian, D., Biochimica et Biophysica Acta - General Subjects, 1860(1), pp. 333-343, 2016 (G) (I) (S)
Role of crystallins in diabetic complications, Reddy, V.S., Reddy, G.B., Biochimica et Biophysica Acta - General Subjects, 1860(1), pp. 269-277, 2016 (G) (I) (S)

2015

Biophysical chemistry of the ageing eye lens, Ray, N.J., Biophysical Reviews, 7(4), pp. 353-368, 2015 (G) (S)
Proteopathies: Biological, molecular and clinical perspectives (Book Chapter), Chowhan, R.K., Dar, T.A., Singh, L.R., Proteostasis and Chaperone Surveillance, pp. 139-169, 2015 (G) (S)
The Effects of Age-Related Modifications on the Stability and Function of Human Eye Lens Crystallins, Ray, Nicholas John, PhD Thesis, Research School of Chemistry, College of Physical and Mathematical Sciences, The Australian National University, 2015 (G)

j.nebel@kingston.ac.uk