Laser Raman
microspectroscopy is a rapid, non-destructive technique
for identifying and analysing small sample volumes (down
to 2µm3) directly under a microscope.
The method is suitable for the identification of solid, liquid and gaseous phases based on their characteristic Raman spectra.
The equipment available is a Renishaw RM1000 Raman spectrometer attached to a research quality Olympus microscope suitable for observations, measurements and image-capture at magnifications up to x 1200.
A computer-controlled x-y-z stage and accompanying imaging software offers the opportunity for 2-d and 3-d Raman mapping of microsamples.
Raman spectra, typically in the range from 4000 to 100cm-1, can be obtained in less than one minute, with a choice of 514nm Ar-ion or 633nm Ne-ion lasers.
An automated heating and freezing stage is also available, allowing analysis over temperatures from -190°C to >300°C (see sheet, "Hot and Cold Stage Microscopy".
Applications include:
Rapid identification of natural minerals and gemstones
Identification of films and layers in samples
Rapid identification of components in synthetic inorganic, organic and biological materials
Speciation of components in liquids and gases
